Figure 3.

H3.3 Knockdown promotes neural progenitor differentiation. (a,b) Timeline of the BrdU birth-dating experiment. Images show brain sections from control or H3.3 knockdown mice were immunostained with an anti-BrdU antibody. The white arrows indicate GFP and BrdU double-positive cells. The scale bar represents 25 μm. The bar graph shows the percentage of BrdU and GFP double-positive cells relative to total GFP-positive cells in the CP (n=3 independent experiments; *P<0.05; bars represent mean±S.E.M). (c–f) H3.3 knockdown increases neuronal differentiation. E17.5 brain sections were stained for TUJ1 (c) or MAP2 (e) after the electroporation of the control or H3.3 knockdown plasmid into the brain at E13.5. The quantification of the percentage of GFP and TUJ1 double-positive cells (d) or GFP and MAP2 double-positive cells (f) relative to the total GFP-positive cells is shown as a bar graph (n=3 independent experiments; *P<0.05; bars represent mean±S.E.M). The scale bar represents 50 μm