Figure 4.

H3.3 Knockdown causes the abnormal neuronal development. (a–c) H3.3 regulates the morphological maturation of neurons in vitro. GFP-positive cells isolated from 2 days post-electroporation brains by H3.3 knockdown (H3.3KD#2) and control were cultured for 72 h. The bar graph shows the number of branch point (b) and the length of the process(c) in vitro. (*P<0.05, bars represent mean±S.E.M). The scale bar represents 5 μm. (d–f) H3.3 regulates the morphological maturation of neurons in vivo at E17.5. The bar graph shows the number of branch point (e) and the length of the process (f) in vivo. (n=11 cells from 3 brains for each condition; *P<0.05, **P<0.01, bars represent mean±S.E.M). The scale bar represents 50 μm. (g–i) H3.3 regulates the neuronal development in vivo at P15. The bar graph shows the number of branch point (h) and the length of the process (I) in vivo. (n=3 independent experiments; **P<0.01, bars represent mean±S.E.M). The scale bar represents 50 μm