Figure 6.

CR synergizes with eEF2K inactivation to inhibit growth of MYCN amplified NB xenografts in vivo. (a) BE(2)-C derived ±eEF2K knockdown tumors were measured by caliper at day 15 and 17 after tumor xeno-transplantation into mice fed with AL or CR diet. After day 17, mice killing was required as tumors in some mice exceeded humane practice guidelines. (b) Analysis of BE(2)-C derived ±eEF2K knockdown tumor growth and mice survival by Kaplan–Meyer plotting (c) in mice fed AL or with CR diet. Mice were killed when tumors reached 1,500 mm³. The sample size for the analysis in the figure is as follows: AL: as in the previous figure. CR: sh-SCR n=8; sh-eEF2K-1 n=7; sh-eEF2K-2 n=8. *P<0.05, **P<0.01, ***P<0.001