Figure 2.

Effect of knockdown and knockup of HNF1b on 3T3-L1 adipocyte differentiation. 3T3-L1 cell lines with stable knockdown (KD) and knockup (KU) of HNF1b were established using lentivirus transfection. HNF1b KD and KU 3T3-L1 cells were induced to adipogenic differentiation. (a and b) BODIPY and Oil Red O staining in differentiated HNF1b KD 3T3-L1 cells with or without 1 μM RSG treatment. Statistical analysis of Oil Red O staining was shown (c). (d and e) BODIPY and Oil Red O staining in differentiated HNF1b KU 3T3-L1 cells with or without 1 μM RSG treatment. Statistical analysis of Oil Red O staining was shown (f). 3T3-L1 cells were transiently transfected with HNF1b plasmid and then induced to adipogenic differentiation. (g) Oil Red O staining in differentiated 3T3-L1 cells transfected with HNF1b plasmid with or without 1 μM RSG treatment. ^#P<0.05