Figure 5.

Knocking down LYAR induces oxidative stress through activating CHAC1 expression in neuroblastoma cells. (a) BE(2)-C cells were transfected with control siRNA, LYAR siRNA-1, CHAC1 siRNA-1, or LYAR siRNA-1 plus CHAC1 siRNA-1, and CHP134 cells were transfected with control siRNA, LYAR siRNA-2, CHAC1 siRNA-2, or LYAR siRNA-2 plus CHAC1 siRNA-2. Seventy-two hours later, the cells were incubated with DCFH-DA for oxidative stress assays. Results were calculated according to fluorescence intensity and expressed as percentage changes, adjusted according to the number of cells and relative to control siRNA-transfected cells. (b) BE(2)-C and CHP134 cells were transfected with control siRNA, LYAR siRNA-1 or LYAR siRNA-2, followed by treatment with NAC at 0, 5, 10 or 20 nM. Seventy-two hours later, the cells were subjected to Alamar blue assays. The numbers of viable cells were expressed as percentage changes. Error bars represented S.E. *P<0.05 and ***P<0.001