Figure 6.

LYAR induces neuroblastoma cell survival by reducing CHAC1 gene expression. BE(2)-C and CHP134 cells were transfected with control siRNA, LYAR siRNA-1, CHAC1 siRNA-1, LYAR siRNA-2, CHAC1 siRNA-2, LYAR siRNA-1 plus CHAC1 siRNA-1, or LYAR siRNA-2 plus CHAC1 siRNA-2 for 72 h. The cells were then stained with propidium iodide and subjected to flow cytometry analyses of the cell cycle (a–d). The percentage of cells at each phase of the cell cycle was calculated and the percentage of cells at the sub-G1 phase was shown in b for BE(2)-C cells and d for CHP134 cells. Error bars represented standard error. ***P<0.001