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. 2005 Mar 10;24(6):1243–1255. doi: 10.1038/sj.emboj.7600596

Figure 3.

Figure 3

TRB3 represses CHOP transactivation. (A) Alignment of CHOP-responsive element in various genes. (B) 293 cells were transiently transfected with the expression plasmids for wild-type or deletion mutants of CHOP fused with GAL4-dbd, pCMV5-Gal4-CHOPs, pFR-luc and pCMV-β-gal. After 48 h, the luciferase activity in cell lysates was measured and was normalized with β-galactosidase activity. (C, D) A375 cells were transiently transfected with p(CHOP)4-Luc, pCMV-β-gal and various combinations of the expression vectors for CHOP and NF-IL6 with or without TRB3. After 48 h, the luciferase activity in cell lysates was measured and was normalized with β-galactosidase activity. (E) 293 cells were transiently transfected with the expression plasmids for Gal4-CHOP(WT) and/or TRB3, pFR-luc and pCMV-β-gal. After 48 h, the luciferase activity in cell lysates was measured and was normalized with β-galactosidase activity. The inset depicts the enlarged figure for the data of GAL4-dbd. (F, G) A375 cells were transiently transfected with p(CHOP)4-Luc, pCMV-β-gal and various combinations of the expression vectors for CHOP and NF-IL6 with or without wild type or deletion mutants of TRB3. After 48 h, the luciferase activity in cell lysates was measured and was normalized with β-galactosidase activity. Similar results were obtained in three independent experiments.