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. 2001 Jul;12(7):1937–1956. doi: 10.1091/mbc.12.7.1937

Figure 2.

Figure 2

(A) 3T3 cell spreading is sensitive to inhibition of a phospholipase A2. Detached cells were suspended in serum-free medium with or without the indicated inhibitor for 15 min and then plated on a FN substrate. Percentage of spreading was evaluated at 60 min. Bars indicate treatment with 30 μM mepacrine [inhib all PLA2 (M)] or 3 μM BPB [inhib all PLA2 (B)], general inhibitors of all PLA2 isoforms; or with AACOCF3 (25 μM), an inhibitor of both iPLA2 and cPLA2 (inhib cPLA2 + iPLA2), or with the iPLA2-specific inhibitor HELSS (10 μM) (inhib iPLA2). All are shown either with (black bars) or without (white bars) 15 μM arachidonic acid (n = 3; means with SEs shown). Mepacrine, BPB, and AACOCF3 all produce dose-dependent spreading inhibition; a single higher concentration of each is shown herein that is reversible with exogenous AA. HELSS treatment produced a small 10–15% reduction at any concentration but the effect was not reversible by addition of AA.