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. 2001 Jul;12(7):1937–1956. doi: 10.1091/mbc.12.7.1937

Figure 3.

Figure 3

(A) Leukotriene B4 reverses spreading blockade from inhibitors of 5-lipoxygenase or cytosolic phospholipase A2. General inhibition of lipoxygenases with 30 μM NDGA (inhib all LOX), and specific inhibition of 5-LOX with 25 μM AA861 (inhib 5-LOX) reduced NIH-3T3 cell spreading, whereas inhibition of either 12-LOX with up to 100 μM baicalein (inhib 12-LOX), or of 15-LOX with up to 25 μM PD146176 (inhib 15-LOX) had no effect. The decrease from NDGA or AA861 was dose-dependent; representative higher concentrations are shown herein. The spreading blockade due to inhibition of any LOX by NDGA or 5-LOX by AA861, or of cPLA2 and iPLA2 by AACOCF3 (inhib iPLA2 + cPLA2) was reversible by addition of the 5-LOX oxidative product 100 nM LTB4 (black bars). Detached cells were incubated with indicated treatment and then plated on FN-coated dishes for 60 min at 37°C (n = 3; means with SEs). (B) Exogenous prostaglandin E2 does not reverse spreading blockade due to phospholipase A2 or lipoxygenase inhibitors. Cells were treated with inhibitors and allowed to spread as in A, except with the cyclooxygenase oxidative product PGE2, at 50 nM (n = 2).