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. 2001 Jul;12(7):1937–1956. doi: 10.1091/mbc.12.7.1937

Figure 6.

Figure 6

(A) Initial transient arachidonic acid release signals most cell spreading. The general PLA2 inhibitor mepacrine (30 μM), added to plated cells at 5 min postplating after the early transient AA release seen in Figure 5A had already occurred, suppressed the second AA release but not the first (bottom; open squares) and still permitted most cell spreading (top; inhib all PLA2 5 min post-plating). Treatment with the same inhibitor before plating suppresses both phases of AA release (bottom; open triangles), and cell spreading (top; inhib all PLA2 pre-plating). (B, top) As with mepacrine in A, 25 μM AACOCF3 (inhib iPLA2 + cPLA2), added to cells after the initial transient AA release at 5 min, blocks the second AA release but not the first, and still permits most cell spreading (bottom) (means and SEs; n = 3). (C) HELSS (10 μM) (inhib iPLA2) added to cells either at 5 min after the initial AA release, or incubated before plating, does not significantly block either phase of AA release (top), or cell spreading (bottom).