Fig. 4. Remifentanil inhibited H₂O₂-induced human fetal osteoblast (hFOB) cell apoptosis.

(A) Morphological changes in hFOB cells were evaluated via Hoechst 33342 staining. The cellular fluorescence changes were observed using a fluorescence microscope. Apoptotic nuclei were seen in the H₂O₂ and 3-methyl A+remifentanil (RPC)+H₂O₂ groups of hFOB cells. In contrast, they markedly decreased in the RPC+H₂O₂ group. (B) The rate of apoptotic cell death was also assessed using a fluorescence-activated cell sorter.