Table 1.
Strains used in this study
| Strains | Genotypea | Reference or source |
|---|---|---|
| DGY505 | MATα suc2-Δ9 trp1 his3 ade2 ura3 leu2 | Gift of Eitan Bibi (Weizmann Institute of Science, Israel) |
| SM1068 | MATα lys1 | Michaelis and Herskowitz (1988) |
| SM1086 | MATα sst2 | Michaelis and Herskowitz (1988) |
| SM1188 | MATa trp1 leu2 ura3 his4 can1 ste14-3∷TRP1 | Sapperstein et al. (1994) |
| SM2515 | [p2μ LEU2 STE14∷HA (Q3)] | Transformant of SM1188 with pSM757 |
| SM2705 | MATα suc2-Δ9 trp1 his3 ade2 ura3 leu2 [p2μ TRP1 PPGK] | Transformant of DGY505 with pRS424-PGK (Schonberger et al. (1996) |
| SM2894 | MATα suc2-Δ9 trp1 his3 ade2 ura3 leu2 [p2μ TRP1 PPGK SUC2] | Transformant of DGY505 with pRS/SUC2 Geller et al. (1996) |
| SM2926 | [pCEN URA3] | Romano et al. (1998) |
| SM3185 | [pCEN URA3 STE14] | Romano et al. (1998) |
| SM3191 | [pCEN URA3 STE14∷HA (V75)] | Transformant of SM1188 with pSM1252 |
| SM3194 | [pCEN URA3 STE14∷HA (K110)] | Transformant of SM1188 with pSM1254 |
| SM3410 | [pCEN URA3 ste14-E213Q] | Transformant of SM1188 with pSM1241 |
| SM3412 | [pCEN URA3 ste14-E214D] | Transformant of SM1188 with pSM1243 |
| SM3413 | [pCEN URA3 ste14-E213D] | Transformant of SM1188 with pSM1244 |
| SM3428 | [pCEN URA3 STE14∷HA (K86)] | Transformant of SM1188 with pSM1256 |
| SM3429 | [pCEN URA3 STE14∷HA (G144)] | Transformant of SM1188 with pSM1258 |
| SM3874 | [pCEN URA3 STE14∷myc (6x) (Q3)] | Transformant of SM1188 with pSM1504 |
| SM3876 | [pCEN URA3 STE14∷myc (6x) (I239)] | Transformant of SM1188 with pSM1506 |
| SM3798 | [p2μ LEU2 STE14∷myc (6x) (Q3)] | Transformant of SM1188 with pSM1472 |
| SM3800 | [p2μ LEU2 STE14∷myc (6x) (I239)] | Transformant of SM1188 with pSM1474 |
| SM4278 | [pCEN URA3 ste14-G31E] | Transformant of SM1188 with pSM1637 |
| SM4279 | [pCEN URA3 ste14-L217S] | Transformant of SM1188 with pSM1638 |
| SM4306 | MATα suc2-Δ9 trp1 his3 ade2 ura3 leu2 [p2μ TRP1 PPGK STE14 (1-25)∷SUC2] | Transformant of DGY505 with pSM1657 |
| SM4307 | MATα suc2-Δ9 trp1 his3 ade2 ura3 leu2 [p2μ TRP1 PPGK STE14 (1-57)∷SUC2] | Transformant of DGY505 with pSM1660 |
| SM4308 | MATα suc2-Δ9 trp1 his3 ade2 ura3 leu2 [p2μ TRP1 PPGK STE14 (1-87)∷SUC2] | Transformant of DGY505 with pSM1658 |
| SM4309 | MATα suc2-Δ9 trp1 his3 ade2 ura3 leu2 [p2μ TRP1 PPGK STE14 (1-239)∷SUC2] | Transformant of DGY505 with pSM1656 |
| SM4321 | [p2μ LEU2 ste14∷HA (Q3)-S148F] | Transformant of SM1188 with pSM1668 |
| SM4322 | [p2μ LEU2 ste14∷HA (Q3)-P173L] | Transformant of SM1188 with pSM1669 |
| SM4324 | [p2μ LEU2 ste14∷HA (Q3)-G132R] | Transformant of SM1188 with pSM1671 |
| SM4326 | [p2μ LEU2 ste14∷HA (Q3)-L81F] | Transformant of SM1188 with pSM1673 |
| SM4361 | MATα suc2-Δ9 trp1 his3 ade2 ura3 leu2 [p2μ TRP1 PPGK STE14 (1-113)∷SUC2] | Transformant of DGY505 with pSM1675 |
| SM4363 | MATα suc2-Δ9 trp1 his3 ade2 ura3 leu2 [p2μ TRP1 PPGK STE14 (1-156)∷SUC2] | Transformant of DGY505 with pSM1677 |
| SM4470 | MATα suc2-Δ9 trp1 his3 ade2 ura3 leu2 [p2μ TRP1 PPGK STE14 (1-239) N191L, P192L∷SUC2] | Transformant of DGY505 with pSM1726 |
a
All strains used in this study are isogenic to SM1188 unless otherwise indicated.