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. Author manuscript; available in PMC: 2018 Jul 24.
Published in final edited form as: Curr Biol. 2017 Jul 6;27(14):2089–2100.e5. doi: 10.1016/j.cub.2017.06.024

Figure 2. Effects of light delivery on neuronal activity and GABA release.

Figure 2

(A) top: There was no effect of light on firing rate following depolarizing current injection in LH neurons not expressing NpHR-eYFP- (eYFP− n=3). However, in NpHR-eYFP+ LH neurons (n=4), introduction of light during depolarizing current injection inhibited firing; bottom: resting membrane potential in NpHR-eYFP- neurons is unchanged by light exposure (−0.22 mV ± 0.25; n=6) whereas resting membrane potential is hyperpolarized in NpHR-eYFP+ neurons (−15.23 mV ± 3.2; n=4), (B) left: averaged data from sample in Figure 2a top; right: averaged data from Figure 2a bottom, (C) Light stimulation of LH GABA terminals produces an optically-evoked IPSC in VTA neurons which is blocked by addition of GABAAR antagonist picrotoxin (PTX), (D) example trace from an optically-evoked IPSC in VTA neurons following LH GABA terminal stimulation that is blocked by PTX. See also Movies S1 and S2.