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. Author manuscript; available in PMC: 2017 Aug 21.
Published in final edited form as: Cell Rep. 2017 Aug 1;20(5):1173–1186. doi: 10.1016/j.celrep.2017.07.021

Figure 1.

Figure 1

Pol II CTD phosphorylation by human TFIIH. (A) Coomassie-stained gel of purified, full-length murine GST-Pol II CTD (GST-CTD). (B) Silver-stained gel of purified human TFIIH. (C) Time course showing TFIIH-dependent phosphorylation of full-length GST-CTD; the single band after 60 minutes suggests a homogeneous phosphorylated state. (D) Western blot corresponding to TFIIH-dependent CTD phosphorylation time course shown in panel C. TFIIH also phosphorylates Ser7, as shown in Figure S3B. See also Figure S1.