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. Author manuscript; available in PMC: 2018 Aug 22.
Published in final edited form as: J Am Coll Cardiol. 2017 Aug 22;70(8):958–971. doi: 10.1016/j.jacc.2017.06.049

Figure 7. Failing Human Cardiac Fibroblasts: Reduced Activation State.

Figure 7

Gallein is shown to reduce the activation state of failing human cardiac fibroblasts. Analysis of (A) collagen gel contraction and (B) cell proliferation by MTT assay in failing human cardiac fibroblasts treated with gallein. *p < 0.05 vs. Veh; **p < 0.01 vs. Veh; ***p < 0.001 vs. Veh; ****p < 0.0001 vs. Veh. (C) Cell migration was measured across a cell-permeable transwell membrane; representative membrane images are shown from which migrated cells were quantified. *p < 0.05. Production of cAMP (D) assessed in normal and failing human cardiac fibroblasts following isoproterenol stimulation. (**p < 0.01 vs. non-failing) and (E) in failing human cardiac fibroblasts treated with or without gallein (*p < 0.05). (F) Representative images of α-SMA immunofluorescence. DAPI = 4',6-diamidino-2-phenylindole; other abbreviations as in Figure 6.