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. Author manuscript; available in PMC: 2017 Aug 21.
Published in final edited form as: Mol Cancer Ther. 2017 Apr 19;16(7):1290–1298. doi: 10.1158/1535-7163.MCT-17-0121

Figure 6. The pro-apoptotic effects of ONC201 in non-TNBC cells involve caspase 8 cleavage, but are TRAIL-independent.

Figure 6

A) Immunoblot analysis of SKBR3 and MCF7 cells treated with a vehicle control or 10μM ONC201 for 72 hours. B) Geometric mean fluorescence intensity of TRAIL and DR5 surface staining was determined in MDA-MB-231 and MCF7 cells treated with a vehicle control or 5 or 10μM ONC201 for 72 hours. C) Annexin-V/PI double positive cells were quantified in SKBR3 using flow cytometry following a 72 hour treatment with a vehicle control or 10μM ONC201 in the presence or absence of 1 ug/mL RIK2 TRAIL blocking antibody. D) Immunoblot of MCF7 cells treated with a vehicle control or 10μM ONC201 in the presence or absence of 1 ug/mL RIK2 TRAIL blocking antibody.