Table 2.

Molecular characteristics of the six patients harboring a PIK3CA mutation

Case ID	Metastatic site	AKT (S473)	P70S6K (T389)	PIK3CA mutation	Mutation description
N02-03-003	Liver	Low	High	E542K	Helical domaina
N02-03-011	Liver	High	High	C420R	Kinase domain
N02-03-012	Liver	High	High	H1047L	Kinase domain
N02-03-020	Liver	High	High	H1047Y, E545K	Kinase, helical domainb
N02-03-037	Liver	Low	Low	N345K	Noncanonical
N02-02-027	Omentum	Low	Low	R1023Q	Unknown role

NOTE: Site of metastasis along with the type of mutation and activation of AKT (S473) and downstream substrate p70S6K (T389) are reported for each patient.

^aConcomitant RPTOR and RPS6KB1 amplification.

^bConcomitant RPS6KB1 amplification.