Figure 2.
Signaling pathway activation mapping of brain metastases from lung cancer by unsupervised hierarchical clustering. Clustering analysis of 128 signaling proteins (horizontal axis) whose activation/expression levels determined by RPMA is shown for 32 patient tumor samples obtained by LCM (vertical axis). In the map, red color represents higher relative levels of activity/expression, black represents intermediate levels, and green represents lower relative levels of activity/expression. (A) Cluster map of all 32 cases with 5 main signaling subgroups indicated (A–E) on the right. (B) Clustering analysis as shown in panel A using the average value for each signaling end point within each subgroup A–E.