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. 2017 Aug 21;3:17053. doi: 10.1038/cddiscovery.2017.53

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Copyright © 2017 The Author(s)

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Nanomotion single-cell cytotoxicity studies. Typical nanomotion response of M17 neuroblastoma cells exposed to (a) monomeric α-syn and (b) the crude mixture of oligomers and fibrils. In both cases, the amyloid species were injected in a two-step procedure involving half dose—1 h stabilization—half dose. The A point in the graph is relative to the last injection. After 4 h from the injection of the α-syn, the monomers do not cause a toxic reaction while the crude mixture had led the cells to death. More than 15 independent repeats were completed for each case. The histograms depict the average variance and the error bars indicate the variability of the variance over the chosen time-step.