Microtubule disruption induced cell-cell adhesion
in NHEK cells. NHEK cells cultured in low calcium media (KGM) were
treated with DMSO (vehicle control) (a, e, and i), nocodazole (b, f,
and j), colcemid (c, g, and k), and cytochalasin B (d, h, and l) for
1 h. The treated cells were visualized by IF staining with
anti-E-cadherin (a–d), anti-β-tubulin (e–h) antibodies and
FITC-conjugated phalloidin for F-actin (i–l). Bar, 20 μm.