Figure 4.

Long-term culture of NHEK in high calcium media (1.2 mM) decreased potential for cell-cell adhesion with nocodazole. (A) Western blot analyses for K10 and loricrin expression were performed with the use of Triton X-100 insoluble fraction (K10) or whole cell lysates (loricrin). NHEKs were cells cultured in low calcium or high calcium media for indicated time. (B) NHEK cells grown in high calcium media for 4 d (top) or 8 d (bottom) were transferred into low calcium media to dissociate cells and then analyzed for nocodazole effects. NHEK cells grown in high calcium media (a and d), low calcium media with DMSO (b and e), and with nocodazole (c and f) were subjected to IF staining with anti-E-cadherin antibody. Bar, 20 μm.