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Expression and knockdown efficiency of BRD4 in GBC cell lines. (A and B) Expression levels of BRD4 in the GBC cell lines NOZ, EH-GB1, GBC-SD, SGC-996 and OCUG were detected by qRT-PCR and western blotting. (C) Immunofluorescence targeting BRD4 (green) and Hoechst 33342 (blue) staining in NOZ and EH-GB1 cells (×200). (D–F) Knockdown efficiency of BRD4 in NOZ and EH-GB1 cells tested by qRT-PCR and western blotting. GADPH was used as the loading control. ***P<0.001.
