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. 2017 Jul 27;51(3):823–831. doi: 10.3892/ijo.2017.4081

Figure 5.

Figure 5

Effect of BRD4 silencing on GBC cell apoptosis. (A and B) Cell apoptosis was evaluated in NOZ and EH-GB1 cells by flow cytometry. Error bars represent the mean ± SD (n=3). (C) Western blotting was used to assess the protein expression of Bad, Bax and Bcl-2. (D) Expression levels of PI3K/AKT and JNK/SAPK pathway proteins were determined by western blotting. (E and F) qRT-PCR and western blotting demonstrated that BRD4 expression level was upregulated in NOZ cells after transfected with BRD4 full length plasmid. (G) Cell growth condition of treated NOZ cells detected by the CCK-8 assay. (H) Protein levels of BRD4, p-AKT and AKT in treated NOZ cells after 72 h using western blotting. ***P<0.001.