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. 2017 Jul 4;51(3):801–811. doi: 10.3892/ijo.2017.4064

Figure 2.

Figure 2

The expression and methylation status of ZNF545 in CRC cell lines and colorectal tissues. (A) Expression of ZNF545 mRNA in CRC cell lines and normal colorectal tissues, and the methylation status of ZNF545 in CRC cell lines. (B) Pharmacological demethylation of ZNF545 by Aza (A) or TSA (T) restored its expression. ZNF545 expression before and after drug treatment was determined by RT-PCR, and demethylation was confirmed by MSP. (C) The methylation status of the ZNF545 promoter in CRC tissues, CRC adjacent tissues, and (D) normal colon tissues. M, methylated; U, unmethylated. (E) ZNF545 expression levels in 9 paired CRC tissues and adjacent tissues were detected by qPCR. CA, colorectal tumor adjacent tissues; CRC, colorectal cancer tissues.