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. 2017 Jul 4;51(3):801–811. doi: 10.3892/ijo.2017.4064

Figure 4.

Figure 4

Re-expression of ZNF545 induces cell cycle arrest and apoptosis of SW480 and HT-29 cells. (A) The effect of cell cycle distribution of vector- and ZNF545-transfected SW480 and HT-29 cells was detected by flow cytometry analysis. Left, representative flow cytometry plots. Right, histograms of cell cycle alterations. (B) Induction of apoptosis detected by flow cytometric analysis with Annexin V-FITC and PI-staining. (C) Quantity analysis of apoptotic cells. Statistical signifcance was assessed by Student's t-test. The graph displays the mean ± SD of three independent experiments. *p<0.05; **p<0.01. (D) Western blot analysis of apoptosis-related proteins in vector-, ZNF545-transfected SW480 and HT-29 cells. β-actin was used as a control.