Figure 6.
AEG-1 interacts with MMP9 in vitro by immunoprecipitation in BCPAP and SW579. (A and B) Immunoprecipitation was performed in BCPAP and SW579 cell lines with either normal IgG or anti-AEG-1 antibody and immunoblotting was performed by anti-MMP2, anti-MMP9 and anti-AEG-1 antibody. (C and D) A reverse IP was performed in BCPAP and SW579 cell lines with either normal IgG or anti-MMP9 antibody and immunoblotting was performed by anti-AEG-1 and anti-MMP9 antibody. Each experiment was performed three times in triplicate.