Tissue	Problem	Possible Reason	Solution
White Adipose Tissue (WAT)	Poor cell dissociation	Poor Digestion	Make sure digestion buffer is at 37°C
	Cell Death	Excessive collagenase digestion	Reduce time of digestion
			Reduce volume of digestion buffer
Aorta	Poor cell dissociation	Poor Digestion	Make sure digestion buffer is at 37 °C
		Aorta pieces in digestion buffer were too large	Make sure aorta is cut into approximately 1 mm pieces
		Aorta pieces were not shaking in dissociation buffer during incubation	Be sure aorta pieces are shaking in the digestion solution
	Cell Death	Excessive collagenase digestion	Reduce time of digestion
			Reduce volume of digestion buffer
Liver	Poor cell dissociation	Poor Digestion	Make sure digestion buffer is at 37 °C
			Increase volume of digestion buffer
		Improper cannulation of IVC (swelling of tissue surrounding IVC occurs)	Be sure needle is properly inserted into the IVC
		Ruptured IVC	Properly secure needle in IVC prior to perfusion
			Reduce perfusion speed
		Ruptured tissue	Reduce perfusion speed
	Cell Death	Improper release of cells from the Glisson’s capsule	Be sure to dissociate cells from capsule using previously discussed stroking method
		Excessive collagenase digestion	Reduce time of digestion
			Reduce volume of digestion buffer