| Condition | Equivalents (EDTA:Ni-PBP-Gly) | pH of EDTA Solution | Overnight Completion |
| 1 | 0 | 4.5 | None |
| 2 | 2 | 4.5 | Partial |
| 3 | 4 | 4.5 | Partial |
| 4 | 6 | 4.5 | Partial |
| 5 | 8 | 4.5 | Full |
| 6 | 10 | 4.5 | Full |
| 7 | 12 | 4.5 | Full |
| 8 | 12 | 5.0 | Full |
| 9 | 12 | 5.5 | Full |
| 10 | 12 | 6.0 | Full |
| 11 | 12 | 6.5 | Full |
| 12 | 12 | 7.0 | Full |
| 13 | 12 | 7.5 | Full |
| 14 | 12 | 8.0 | Partial |
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