Solution 1: 1 M of ammonium acetate. | |
Component | Amount for 50 mL |
Ammonium acetate | 3.86 g |
Water (make up to 50 mL) | ~ 50 mL |
Solution 2: 100 mM of ammonium acetate. | |
Component | Amount for 50 mL |
Solution 1 | 5 mL |
Water | 45 mL |
Solution 3: 10% Sodium deoxycholate. | |
Component | Amount for 1 mL |
Sodium deoxycholate | 0.1 g |
Water | 1 mL |
Solution 4: SDC homogenization buffer (100 mM ammonium acetate containing 1% Sodium deoxycholate). | |
Component | Amount for 10 mL |
Solution 2 | 9 mL |
Solution 3 | 1 mL |