B. Multipotent Differentiation.

Sample	Tissue	Adipocyte	Osteoblast	Chondrocyte
mCRPC-1-3c	Lymph Node	+	+	+
mCRPC-2-1c	Bone	+	+	+

(A) Percent of cells defined as MSCs by flow cytometry in metastatic lesions from mCRPC patients undergoing a rapid autopsy at the time of death (mean: 0.1%; median: 0.002%). MSCs could be detected in 50% of mCRPC lesions from distinct organ sites. (B) MSCs expanded from mCRPC lesions obtained from distinct organ sites in independent patients at the time of death have tri-lineage differentiation potential (i.e. adipogenesis, osteogenesis, and chondrogenesis).