Figure 7. Conditioned medium from CAFs overexpressing the nuclear GPER P16L variant induces the migration of MDA-MB-231 breast cancer cells.

A. Representative micrographs of Boyden chamber assays performed with MDA-MB-231 cells treated with conditioned medium (CM) from CAFs. CAFs_I were transfected for 24 hours with control shRNA or shGPER constructs or a P16L mutant version of the GPER expression vector (FLAG GPER P16L) and then treated for 18 hours with 100 nM E2 where indicated. B. Quantitation of the Boyden chamber assays shown in panel A. Values represent the mean ± SD of the number of migrated cells counted in 10 random fields. Data are representative of two independent experiments performed with triplicate samples.