Table 1. Association of immunological and clinicopathological factors with survival parameters (univariate).

		Overall	Events (OS)	Mean overall survival (SE)	p-value	Events (DSS)	Mean disease specific survival (SE)	p-value	Events (DFS)	Mean disease free survival (SE)	p-value
		125	79	76.9 (7.1)		65	89.0 (7.9)		53	86.6 (7.4)
Age					0.447			0.401			0.710
	median and below	68	45	77.9 (8.9)		36	89.6 (10.0)		33	82.4 (8.7)
	above median	57	35	57.6 (6.6)		29	65.3 (7.0)		20	77.4 (7.4)
Sex					0.140			0.067			0.192
	male	95	64	73.1 (8.0)		54	82.3 (8.8)		43	82.4 (8.2)
	female	30	16	72.4 (8.3)		11	83.7 (8.6)		10	83.8 (8.8)
pT					<0.001			<0.001		mean DFS not reached	0.002
	1	55	26	106.2 (11.5)		31	119.0 (12.2)		15
	2	40	36	38.9 (5.5)		29	44.9 (6.5)		25
	3	28	17	76.8 (13.2)		3	86.8 (14.0)		13
	4	2	1	36.9 (17.7)		2	36.9 (17.7)		0
pN					0.012			0.014		mean DFS not reached	0.005
	0	68	38	82.8 (8.6)		42	92.9 (9.2)		24
	1	47	37	60.7 (9.5)		34	74.0 (11.6)		23
	2	8	3	63.9 (14.0)		3	63.9 (14.0)		4
	3	2	2	13.9 (4.8)		2	13.9 (4.8)		2
pM					0.001			<0.001			<0.001
	0	121	76	79.0 (7.2)		61	91.6 (8.1)		49	89.4 (7.5)
	1	4	4	6.0 (2.8)		4	14.5 (6.0)		4	8.9 (5.6)
UICC Stage					0.008			0.002			<0.001
	1	51	21	66.6 (6.8)		19	74.7 (7.2)		23	85.7 (7.2)
	2	49	24	76.8 (10.1)		24	85.6 (11.0)		25	79.5 (9.7)
	3	21	37	45.1 (7.9)		34	55.6 (8.8)		36	64.1 (9.6)
	4	4	4	14.5 (16.0)		4	14.5 (6.0)		4	8.9 (5.6)
Grade (WHO)					0.039			0.071			0.402
	1	6	1	143.2 (19.0)		1	143.2 (19.0)		2	114.2 (24.7)
	2	61	39	81.2 (9.9)		31	94.3 (11.2)		28	85.2 (9.6)
	3	58	40	52.5 (6.0)		33	60.1 (6.6)		53	69.6 (7.5)
CD3i					0.019			0.050			0.009
	low	81	55	62.1 (7.4)		44	73.9 (8.6)		39	78.1 (8.9)
	high	44	25	96.4 (11.9)		21	106.1 (12.5)		14	104.9 (11.1)
Distribution of CD3i					0.007			0.003			0.004
	focal	75	54	58.3 (7.1)		46	65.5 (7.8)		37	73.4 (8.9)
	diffuse	50	26	100.1 (11.8)		19	118.7 (12.6)		16	106.5 (11.1)
CD8i					0.163			0.486			0.185
	low	87	58	73.3 (8.4)		45	90.3 (9.6)		39	81.5 (8.7)
	high	38	22	83.5 (10.7)		20	88.0 (11.0)		14	100.3 (12.2)
Distribution of CD8i					0.015			0.080			0.030
	focal	85	60	61.6 (8.0)		47	74.6 (10.3)		40	70.8 (6.9)
	diffuse	40	20	94.1 (11.0)		18	98.7 (11.1)		13	110.1 (11.7)
PD1i					0.035			0.117			0.169
	low	84	53	60.6 (7.4)		42	72.2 (8.7)		35	80.8 (9.3)
	high	41	27	95.5 (12.5)		21	103.2 (13.0)		18	88.9 (9.0)
PD-L1+ TILs					0.870			0.815			0.844
	low	89	56	77.2 (8.6)		47	87.2 (9.5)		38	82.8 (8.4)
	high	36	23	69.6 (10.7)		18	84.1 (12.4)		15	96.4 (12.8)
PD-L1 TCs					0.028			0.006			<0.001
	low/absent	87	54	65.0 (8.8)		47	71.7 (9.7)		42	62.4 (6.8)
	high	38	26	87.8 (9.1)		18	103.8 (10.6)		11	122.4 (10.6)
Staining intensity of PD-L1					0.720			0.883			0.293
	weak	30	18	55.9 (8.1)		13	68.2 (9.0)		14	60.9 (9.2)
	intermediate	31	15	73.9 (11.6)		12	97.9 (14.3)		12	117.6 (13.3)
	strong	28	20	87.1 (13.3)		17	96.1 (12.8)		8	96.8 (14.2)
PD-L1 copy number status					0.781			0.533			0.951
	Amplification	3	2	25.4 (15.1)		2	25.4 (15.1)		1	37.2 (17.4)
	Polysomy	19	6	66.2 (10.3)		3	66.2 (10.3)		4	69.2 (11.4)
	Disomy	92	61	76.5 (8.1)		49	89.2 (9.0)		39	88.5 (8.3)
	Deletion	10	11	52.5 (10.0)		11	64.4 (10.0)		9	56.5 (11.4)
CD3i/PD-L1 subgroups					0.031			0.012			0.001
	low/low	60	44	46.8 (5.3)		38	51.6 (5.8)		33	53.8 (6.3)
	low/high	21	11	76.9 (7.1)		6	117.4 (16.0)		6	113.9 (16.8)
	high/high	27	15	86.4 (16.0)		12	100.6 (12.8)		5	130.3 (12.7)
	high/low	17	10	95.4 (21.4)		9	101.4 (22.0)		9	77.4 (14.7)