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. 2017 May 23;8(29):46781–46800. doi: 10.18632/oncotarget.18108

Figure 1. Overexpressed CENP-A mislocalizes to chromosome arms in human cells.

Figure 1

A. Constitutive overexpression of CENP-A leads to mislocalization to chromosome arms. Representative chromosome spread images of HeLa YFP-CENP-A cells immunostained with antibody against GFP to visualize YFP-CENP-A and stained with DAPI for DNA. Insets correspond to boxed area in the main image. Scale bar: for insets. B. Inducible expression of CENP-A leads to mislocalization to chromosome arms. Representative chromosome spread images of HeLa FRT/TO mCherry-CENP-A cells treated with indicated concentrations of tetracycline. Cells were immunostained with antibody against CENP-A and stained with DAPI for DNA. Scale bar: 2 μm. C. CENP-A levels increased at non-centromeric regions but not at centromeres in CENP-A overexpressing cells. Prism graphs show signal intensities of CENP-A at centromeres (left) and non-centromeric regions (right) in chromosome spreads of HeLa FRT/TO mCherry-CENP-A cells treated with indicated concentrations of tetracycline. Each circle represents a spot and ‘n’ denotes number of spots analyzed. Red horizontal lines represent mean signal intensity as indicated. Error bars represent standard error of mean (SEM) across areas measured. P-values calculated using the Mann-Whitney U test are indicated.