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. 2016 Apr 12;8(29):46801–46817. doi: 10.18632/oncotarget.8695

Figure 5. A low concentration of SC-2001 (2.5 μM) combined with ABT-737 inhibited the self-renewability of MICs.

Figure 5

Secondary sphere assays with the melanoma cells A. Bright-field images of secondary spheres. Scale bar = 100μm B. Quantification of the number of secondary spheres. The combination treatment significantly decreased the number of secondary spheres formed compared with DMSO, ABT-737 or SC-2001 treatment alone (P < 0.05 or less) in eight out of nine lines tested. The p-values for all comparisons are separately listed in Supplementary Table S4. C. Visualization of the secondary sphere cells with the Ethidium Bromide/Acridine Orange (EtBr/AO). Scale bar = 100μm. The cells were not treated with any drugs during the secondary sphere assay.