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. 2016 Apr 12;8(29):46801–46817. doi: 10.18632/oncotarget.8695

Figure 9. Knock-out of BIM protected melanoma cells against combination-induced cell death.

Figure 9

WM852c and HT144 melanoma cell lines with BIM knockout were generated with the CRISPR/Cas9 system. Knockout of BIM were determined by Immunoblots A. and B. and these cells were used for sphere assays C. and D. (B) also shows the full length and cleaved PARP from the HT144 cells, with either control or CRISPR-mediated knockout of BIM, upon indicated treatments in sphere conditions. The ratio of Cleaved/full length PARP of the cells treated with combination was quantified, and the one in the control cell was set as 1. (C) and (D) show the sphere assays with the knockout clones (KO1 and KO2) of WM852c cells with indicated drug treatments for 48 hrs. KO of BIM significantly protected the cells against the combination-induced disruption of spheres (P<0.05 or less) in both WM852c and HT144. **indicates P<0.01; *indicates P<0.05.