Figure 1. Reappraisal of transcriptome dataset in urothelial carcinoma (GSE31684).

Clustering analysis of genes focusing on those involving heparan sulfate proteoglycan metabolic process revealed SULF1 is the most significantly up-regulated gene associated with increments of pT status, followed by SULF2, prompting us to further validate their significance. Tissue specimens from tumors with different pT statuses are indicated on top of the heatmap, and expression levels of up-regulated and down-regulated genes are represented as a spectrum of brightness or red and green, respectively. Cases unaltered in mRNA transcriptional level are coded black.