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. 2017 Apr 12;8(29):47250–47268. doi: 10.18632/oncotarget.16872

Figure 4. GBK induces cell cycle arrest at G1/S phase by downregulation of RNA and proteins important for G1-S phase transition in cancer cells.

Figure 4

(A) Differential expression of genes in MCF-7 cells after treatment with GBK was analyzed by RT-qPCR. Genes related to replication and cell cycle were downregulated, while the tumor repressor gene p53 was upregulated. (B) Effect of GBK on the expression of cell cycle-related proteins in MCF-7 cells in dose-dependent and time-dependent experiments. MCF-7 cells were treated with various concentrations of GBK (0–580 μg/ml) for 48 h, or 290 μg/ml GBK in a time course. Protein lysates were analyzed by western blot. β-tubulin was used as an internal control. (C) Effect of GBK on G1 cell cycle regulatory complex formation. MCF-7 cells were treated with 290 μg/ml of GBK for 48 h. Total cell lysates were immunoprecipitated with anti-CDK2 antibody and analyzed by western blot analysis using anti-p27 and anti-CDK2 antibodies.