Figure 1. Fluoro-substituted STn vaccines induce efficient antitumor immunotherapy in the presence of adjuvant.
(A) Preparation of the glycoconjugates. (B) Schematic representation of the survival study design and immune scheme. One day before the immunization schedule, mice were given intravenous (i.v.) injection of cyclophosphamide (CY) at a dose of 100 mg/kg per mouse. The glycoconjugates in the presence of adjuvant were immunized five times at biweekly intervals and animals were challenged via the tail vein 7 days after the 4th immunization with 2 × 105 CT-26 cells. (C) Survival time of tumor-bearing mice. KLH group (n = 8), 1-KLH group (n = 8), 2-KLH group (n = 8), 3-KLH group (n = 7) and 4-KLH group (n = 7). There is significant difference between 4-KLH group and 1-KLH group. The abscissa represents the time after tumor inoculation.