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. 2017 May 7;8(29):47330–47343. doi: 10.18632/oncotarget.17646

Figure 3. Fluoro-substituted STn vaccines improve the antibody response in the presence of adjuvant.

Figure 3

1-KLH, 2-KLH, 3-KLH and 4-KLH in the presence of adjuvant were immunized five times at biweekly intervals and animals were challenged via the tail vein 7 days after the 4th immunization with 2 × 105 CT-26 cells. The mice were bled by tail vein on day 13 after the third immunization on day 14 after the fifth immunization. (A–E) ELISA tests of anti-STn antibody titers after 5th immunization with antigen. Each data point represents the titer of an individual mouse and the horizontal lines indicate the median for the group of mice. (F) IgG subtypes after immunized with 1-KLH, 2-KLH, 3-KLH and 4-KLH by ELISA with a 1:1000 dilution of pooled sera. The pooled sera obtained from 13 days after the 3rd vaccination. OD490 = optical density at 490 nm. The results in (A–E) are representative of three independent experiments. The results in (F) represent the mean ± standard error of measurement (SEM) of at least two experiments.