Table 1. Immunological results after vaccination with synthetic carbohydrate conjugates in the presence of adjuvant.

Vaccine	ELISA titer anti-STn				ELISA titer anti-modified-STn
	After third		After fifth		After fifth
	IgG	IgM	IgG	IgM	IgG
KLH	< 100	< 100	< 100	< 100	------
1-KLH	15210	< 100	347080	126	347080
2-KLH	128672	< 100	444915	< 100	770080
3-KLH	< 100	< 100	14479	< 100	34026
4-KLH	532951	< 100	1047799	< 100	1739261

KLH, 1-KLH, 2-KLH, 3-KLH and 4-KLH in the presence of adjuvant were immunized five times at biweekly intervals and animals were challenged via the tail vein 7 days after the 4th immunization with 2 × 10⁵ CT-26 cells. ELISA anti-STn or anti-modified-STn antibody titers in the presence of adjuvant were determined by coating ELISA plates with the 1-BSA or modified-STn-BSA. Using the pooled antisera of all immunized mice obtained from 13 days after the third immunization or 14 days after the fifth immunization, and the antibody titer was defined as the highest dilution showing an absorbance of 0.1, after subtracting background. Anti-STn IgM titers could not be detected when diluted 1:100 (expressed as < 100). The results are representative of three independent experiments.