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. 2017 Apr 18;8(29):47344–47355. doi: 10.18632/oncotarget.17185

Figure 2. Expression of Rain, Gtl2 and Rtl1 in AN and AI cell lines.

Figure 2

(A, B, and C) Quantitative PCR analysis for Rain, Gtl2 and Rtl1, which are in the Dlk1-Dio3 region, in AN and AI lines. AN F: full reprogrammed cells as shown by tetraploid complementation assay in AN lines; AN P: partially reprogrammed cells as shown by tetraploid complementation assay in AN lines; AI F: full reprogrammed cells as shown by tetraploid complementation assay in AI lines; AI P: partially reprogrammed cells as shown by tetraploid complementation assay in AI lines. Relative mRNA expression is normalized to GAPDH and is represented relative to expression in R1 ESCs. The experiments were performed in triplicate (means±SD; n=3; Student's t test; *p<0.05; **p<0.01, and ***p<0.001). The primers used are listed in Supplementary Table 2.