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. 2017 May 7;8(29):47412–47424. doi: 10.18632/oncotarget.17658

Figure 4. 5-HT2BR promoted the proliferation, invasion and metastasis of HepG2 and HHCC cells.

Figure 4

(A) The expression of 5-HT receptors in different types of hepatoma cells. The 5-HT receptors expression of SMMC-7721 were considered as 1. (B–C) 5-HT promoted the expression of 5-HT2BR in HepG2 and HHCC at the protein (B) and mRNA levels (C). (D) Inhibition of 5-HT2BR inhibited the promotion effect on proliferation induced by 5-HT. (E–F) Inhibition of 5-HT2BR reduced the S+G2/M phase cell ratio in HepG2 and HHCC cells (E), and cell cycle marker proteins were also detected (F). (G) 5-HT administration and 5-HT2BR inhibition did not affect cell apoptosis. (H) Inhibition of 5-HT2BR induced slower wound closure in HepG2 and HHCC cells, quantified by the healing rate. (I) Inhibition of 5-HT2BR induced less cell penetration, quantified by the number of penetrated cells. Blue asterisk: 5-HT group vs 5-HT+SB204 10 μM; Green asterisk: 5-HT group vs 5-HT+SB204 100 μM; ns: no statistical significance, *P < 0.05, **P < 0.01.