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. 2017 May 10;8(29):47607–47618. doi: 10.18632/oncotarget.17744

Figure 4. Nac1 OE delays but cannot block ESC differentiation.

Figure 4

(A) Immunoblot analysis after Nac1 OE. (B) Morphology of colonies formed by the indicated lines. Cells were grown with or without LIF for 6 days. Scale bar, 100 μm. (C) AP staining of colonies formed by plating the indicated cells at clonal density and culturing for 6 days with or without LIF. Percentage of colony types (undifferentiated, mixed, or differentiated) formed by cells is shown. (D) The indicated cells were cultured for 5 days with or without LIF and cell numbers were counted. (E, F) qRT-PCR analyses of pluripotency (E) and germ layer (F) marker expression levels in the indicated lines after 0, 3 and 6 days of EB differentiation. All data are normalized to Gapdh and shown relative to WT ESCs (set at 1.0). Data in (C–F) are represented as means ± s.d.; n = 3. *p < 0.05; **p < 0.01. All P-values were calculated using Student's t-test.