Figure 1. Penfluridol suppresses glioblastoma tumor growth and associated MDSCs.
1×106 U87MG cells in 1:1 mixture of PBS and matrigel were implanted in right and left flanks of 4-6 week old athymic nude mice. Treatment with 10mg/kg penfluridol by oral gavage everyday started once tumor size was 70-100mm3 after tumor cells injection till day 48. Tumors from all control and penfluridol treated mice were removed at day 48. (A) Bar graph representing average tumor volume of control and penfluridol treated mice at day 48. Values were plotted as mean ± SEM. (B-C) Representative images of tumors as well as mice bearing tumors from control and treated group. Peripheral blood mononuclear cells (PBMCs) were collected from the blood obtained from control and penfluridol treated U87MG tumor-bearing athymic nude mice. Modulation of CD11b+ and Gr-1+ cells was analyzed by immunostaining and fluorescence cytometry to determine the effects of penfluridol on mouse myeloid derived suppressor cells (MDSCs); statistical analysis was performed by Student's t test. (D) Bar chart showing percent MDSCs which were double stained with CD11b and Gr-1 mouse antibodies. *Statistically significant at p<0.05 when compared with control.