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. 2017 Apr 26;8(29):47632–47641. doi: 10.18632/oncotarget.17425

Figure 4. Elevation of M1 macrophages in tumor with penfluridol treatment.

Figure 4

Subcutaneously implanted U87MG glioblastoma tumors in SCID-NOD mice were removed from control and penfluridol treated mice at day 40. Tumors were processed and suspended into single cell suspension with the help of tumor dissociator kit and use of gentle MACS dissociator. Effect of penfluridol treatment on macrophages was analyzed by immunostaining and florescence cytometry. (A) Bar graph representing percent monocytic cells double stained with CD86 and IL12 human specific antibodies. (B) Percent cells which were single stained with CD88 human specific antibody.

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