(A) Serum protein levels of IL-1β, IL-6, IL-4 and IL-10 after mice with LPS or saline intraperitoneal injection (n=6). (B) Measurement of serum αMSH level before and after LPS or saline intraperitoneal injection (n=6). Epididymis white adipose tissue was isolated after intraperitoneal injection with LPS or saline, then mRNA levels of MC5R
(C), Foxo1, Foxo3a, Foxo4 and Foxo6
(D) were detected (n=6). Base on the LPS/saline injection, mice were injected with another 500 nM αMSH, then further detected for relative serum protein levels of IL-1β, IL-6, IL-4 (E) and mRNA levels of M1, M2 markers (F), MC5R
(G), IL-6, MCP-1, Leptin
(H), Foxo1, Foxo3a, Foxo4 and Foxo6
(I) in white adipose tissue (n=6). Values are means ± SD. vs. control group, * P < 0.05.