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. 2017 Apr 27;8(29):47642–47654. doi: 10.18632/oncotarget.17465

Figure 5. Foxo4 negatively regulate MC5R transcription in αMSH inhibited inflammation in mice adipocytes.

Figure 5

(A) Fragments of MC5R promoter fused to a luciferase reporter plasmid or PGL3-basic (control) were co-transfected into cells together with Renlilla plasmid and pAd-Foxo4 (n=3). Luciferase activity was corrected for Renilla luciferase activity and normalized to control activity (n=3). (B) Chromatin immunoprecipitation (ChIP) analysis of Foxo4 and MC5R interaction. (C, D) After pAd-Foxo4 together with αMSH or pc-MC5R in LPS/saline treatment, MC5R mRNA level was determined in adipocytes (n=3). (E) When adipocytes were treated with pAd-Foxo4 and αMSH in LPS/saline treatment, mRNA levels of Foxo4, IL-6, TNFα, MCP-1 and Leptin were analyzed (n=3). Values are means ± SD. vs. control group, * P < 0.05.