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. 2017 May 2;8(29):47675–47690. doi: 10.18632/oncotarget.17544

Figure 6. ATP6V1C1 enhances human breast cancer proliferation in cell lines with high and low metastatic potential through mTOR pathway activation stimulated by amino acids.

Figure 6

The effects of knockdown of ATP6v1c1 were examined in cells with low, MCF-7 (A-C), and high, MDA-MB-435s (D-F) metastatic potential. MCF-7 cells: A. Representative data of anti-BrdU staining of MCF-7 cells treated with different lentiviruses as indicated after 3 hours incubation with BrdU (Red arrows show the BrdU positive cells. Blue arrows show the BrdU negative cells). B. Quantification of percentage of BrdU positive cells per view. (n=10). Results are mean ± s.e.m. *P<0.05, **P<0.01, ***P<0.001. C. Representative data of p-p70S6K, p-AKT, p-ERK1/2 and ATP6V1C1 expression in MCF-7 cells treated with different lentiviruses with or without amino acid stimulation as indicated by Western blotting. MDA-MB-435s cells: D. Representative data of anti-BrdU staining of MDA-MB-435s cells treated with different lentiviruses as indicated after 3 hours incubation with BrdU (Red arrows show the BrdU positive cells. Blue arrows show the BrdU negative cells). E. Quantification of percentage of BrdU positive cells per view. (n=10). Results are mean ± s.e.m. *P<0.05, **P<0.01, ***P<0.001. F. Representative data of p-p70S6K, p-AKT, p-ERK1/2 and ATP6V1C1 expression in MDA-MB-435s cells treated with different lentiviruses with or without amino acid stimulation as indicated by Western blotting.