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. 2017 May 9;8(29):47709–47724. doi: 10.18632/oncotarget.17711

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Copyright: © 2017 Yang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A) RT-PCR showing NDRG1 mRNA levels in 16 selected samples from downstaging and no-downstaging group respectively. Samples were selected by random numbers generated with SAS software. (B) Western blot analysis of NDRG1 expression in selected CRC samples. (C) NDRG1 protein levels in tissues from patients of downstaging and no-downstaging groups were measured with IHC staining. (D, E) CRC patients with negative expression of NDRG1 presented poorer overall survival (D) and disease free survival (E) compared with those with positive expression of NDRG1. Bars indicated SD. *, P < 0.05, **, P < 0.01.