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. 2017 May 9;8(29):47709–47724. doi: 10.18632/oncotarget.17711

Figure 3. NDRG1 induced degradation of Bcl-2 via ubiquitin-proteasome pathway.

Figure 3

(A) HCT116 and SW620 cells pretreated with with CHX (20μM, 6h), a classical protein synthesis inhibitor, were transfected with NDRG1. Protein levels of Bcl-2 were analyzed with western blots 24 hours later. (B) After treated with lysosomal inhibitor BafilomycinA1 (BafA, 0.2μM), protein levels of Bcl-2 were analyzed in control and NDRG1-transfected cells. (C, D) Western blots showing accumulation of Bcl-2 protein after treatment with MG132 (10μM) in NDRG1-transfected cells. All histograms showed mean values from three independent experiments; bars indicated SD. *, P < 0.05, **, P < 0.01.